We recommend you an ELISA data analysis software curve expert 1.3 or curve expert 1.4 that is simple to use and you can download at the end of this page to process data calculation. This ELISA standard curve protocol will give you a comprehensive instruction on how to calculate ELISA results with quantitative ELISA kit step by step.įirstly, you need to run the ELISA kit according to our guide of plate layout and get raw data from ELISA reader, and then let's go to the next step-statistical analysis of ELISA results, which is the last and the key step to get the concentration of target protein in your samples.

When you analyze ELISA data with quantitative ELISA kit, you have to plot the mean absorbance against the protein concentration and draw a curve fitting your standard result best, and then interpolate absorbance of samples to the curve to calculate the concentration. Generally speaking, with quantitative ELISA kit, you can quantify target protein in samples since you test a serial of standard proteins of known concentration. However, we can't calculate the exact concentration since there isn't any standard protein in the kit. Semi-Quantitative: With this type of ELISA kit, we are able to get a negative or positive result as well as to compare the target protein levels in assay samples, since the level of samples'absorbance will directly correspond to the level of target protein concentration. Qualitative:we simply get a negative or positive result from the assay to determine whether there is any target protein existing in a certain sample by comparing to the negative control. ELISA data of samples can be interpolated from standard curve to calculate the concentrations of target proteins in samples. Quantitative: A standard curve can be generated by ODs of serial diluted standard proteins and corresponding known concentrations.

Three types of ELISA data output can be yielded. ELISA assay is used for determining the concentration of target protein in biological samples.

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